Mammalian\Insect cells哺乳动物和昆虫细胞破碎

Our versatile Homogenizer systems provide efficient cell disruption of both insect and mammalian cells with controllable pressures and temperature management systems - both of which help protect intracellular contents quality including proteins.

Mammalian cells

Mammalian cell cultures are commonly used because of their genetic modifiability and ease of maintenance. With their thin membrane they are an easy cell type to disrupt. Milder conditions during cell disruption can be provided by our adaptable technology which will preserve the quality and yield of the cell contents and to leave them undamaged.

In some projects detergent-based cell lysis is used first to break apart the lipid bilayer and then mechanical homogenizers (like ours), operating at lower pressures, complete the cell breakage so that all cell contents of interest can be obtained.

Insect cells

Insect cells used in conjunction with the baculovirus expression vector system (BEVS) is gaining in popularity as a platform for recombinant protein production.

Use of our high pressure technology may offer advantages. One example is the utilization of our modern 'French Press ©' Cell Homogenizer to disrupt Sf9 insect cells to generate a lysate for use in protein purification.

Our High Pressure Homogenizers

Improved yield of cell contents, efficiency and easier downstream processing are all potential benefits.

Sophisticated temperature control both pre-processing, during and after cell disruption.

Adjustable pressure from 1,500 psi (100 bar) to 60,000 psi (4200 bar) for efficient processing.

Easy to scale systems from Lab R&D samples to full production systems with volumes in excess of 1000 l/h.

Easy to use, maintain, and sterlize with low ongoing ownership costs.

Lab Cell Homogenizer

Homogenizer for mammalian cells.

Pilot - Production Homogenizer

Homogenizer for insect cells - Sf9.

我们的多功能高压均质器系统通过可控的压力和温度管理系统，为昆虫和哺乳动物细胞提供高效的细胞分解，这两种系统都有助于保护细胞内内容物的质量，包括蛋白质。

哺乳动物细胞

哺乳动物细胞培养物因其基因可修改性和易于维护而被广泛使用。由于细胞膜很薄，它们是一种容易被破坏的细胞类型。在细胞分裂期间，我们的适应性技术可以提供更温和的条件，这将保持细胞内容物的质量和产量，并使它们不受损害。

在一些项目中，首先使用以净化剂为基础的细胞裂解来打破脂质双分子层，然后使用机械均质器(像我们的)，在较低的压力下操作，完成细胞破碎，以便获得所有感兴趣的细胞内容物。

昆虫细胞

与杆状病毒表达载体系统(BEVS)结合使用的昆虫细胞作为重组蛋白生产的平台越来越受欢迎。

使用我们的高压技术可能会带来好处。一个例子是利用我们的现代“French Press©”细胞均质器来破坏Sf9昆虫细胞，产生用于蛋白质纯化的裂解液。

高压均质机

提高细胞内容物的产量，效率和更容易的下游处理都是潜在的好处。

复杂的温度控制，包括预处理过程中和细胞分裂后。

可调压力从1500 psi (100 bar)到60,000 psi (4200 bar)的高效处理。

易于将系统从实验室研发样品扩展到容量超过1000升/小时的完整生产系统。

易于使用，维护，消毒与低持续拥有成本。

实验室细胞均质器

哺乳动物细胞的均质器。

中试生产均质机

昆虫细胞均质器- Sf9。