Yeast Cells酵母菌破碎

Our technology provides the versatility and controllable high pressures for efficient cell disruption of even the toughest yeast cells like Pichia Pastoris. Other cells including Saccharomyces cerevisiae are disrupted with equal efficiency and for temperature sensitive proteins we have sophisticated cooling systems to protect intracellular contents purity and quality.

However, access to yeast cell contents, including proteins, can be challenging due to the rigid and protective cell structures of some yeasts (and fungi). Our technology can help meet these challenges.

Yeast Cells - Our High Pressure Homogenizers

 Controllable process pressure from 1,500 psi (100 bar) to 60,000 psi (4200 bar) - the highest available for HPH.

 Sophisticated for temperature control pre-processing, during and after homogenization.

 Variable flow rates from 1m to 275ml per minute in the Lab -> 16 l/h to 130 l/h to 1000 l/h at production scale.

 Easy to use and sterlize with low ongoing operating costs.

 HPH pretreatment can accelerate S. cerevisiae yeast autolysis which may result in higher protein, amino acid and total solids yield.

With yeasts cells that require higher pressures there is often a balance of whether to go to with a high pressure for more disruption in a single pass or to multi pass at lower pressure. Higher pressure results in higher temperature which may, in some cases, be detrimental to protein recovery (which can be counteracted by our cooling systems). We can help advise on HPH processing parameters for your appliation.

我们的技术提供了多功能性和可控的高压，即使是最坚韧的酵母细胞，如毕赤酵母，也能有效地破坏细胞。包括酿酒酵母在内的其他细胞以同样的效率被破坏，对于温度敏感的蛋白质，我们有复杂的冷却系统来保护细胞内内容物的纯度和质量。

然而，由于一些酵母(和真菌)的刚性和保护性细胞结构，获取酵母细胞内容物，包括蛋白质，可能具有挑战性。我们的技术可以帮助应对这些挑战。

酵母细胞-我们的高压均质机

可控制的过程压力从1500 psi (100 bar)到60,000 psi (4200 bar) -最高可用于HPH。

复杂的温度控制预处理，期间和后均质。

实验室中每分钟1m至275ml的可变流量-> 16升/小时至130升/小时至1000升/小时的生产规模。

易于使用和消毒，持续运营成本低。

HPH预处理可促进酵母自溶，从而提高蛋白质、氨基酸和总固形物的产量。

对于需要更高压力的酵母菌细胞，通常需要平衡是用高压进行单次传代，还是在较低的压力下进行多次传代。更高的压力导致更高的温度，在某些情况下，这可能不利于蛋白质的恢复(这可以通过我们的冷却系统抵消)。我们可以为您的申请提供HPH加工参数方面的建议。

Lab Scale Cell Disruptor - modern 'French Press' type.

Production Scale